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. 2018 Dec 20;9:658. doi: 10.3389/fgene.2018.00658

FIGURE 2.

FIGURE 2

Impaired Nrf2 activation in primary RPE cells isolated from XBP1 KO mice. Primary RPE cells were isolated from WT and KO mice and cultured in a 12-well plate until confluence. (A) Phase contrast image of isolated RPE cells. (B) After proteasome inhibitor MG132 treatment (10 μM, 30 min), Nrf2 expression in primary RPE cells was detected by Western blot and semi-quantified by densitometry (n = 3 independent isolations, ∗∗p < 0.01). (C) Primary RPE cells were treated with a potent pro-oxidant, hydroquinone (HQ) (100 μM, 6 h), and Nrf2 expression was detected by Western blot (n = 3 independent isolations; ∗∗∗p < 0.001 vs. XBP1+/+ untreated control, ‡p < 0.05 vs. XBP1 +/+ cells treated with HQ).