Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 20;9:658. doi: 10.3389/fgene.2018.00658

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Chen, Zhong, Wang, Yu, Plafker, Plafker and Zhang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Overexpression of Nrf2 does not alter XBP1 expression in ARPE-19 cells. (A) ARPE-19 cells were transfected with adenovirus overexpressing Nrf2 or LacZ as control for 24 h, mRNA level of HO-1, spliced XBP1 and total XBP1 was determined by real-time RT PCR (mean ± SD, n = 3, ^∗∗p < 0.01 vs. ad-LacZ transfected cells). (B) ARPE-19 cells were transfected with adenovirus overexpressing Nrf2 or LacZ for 48 h, Nrf2, unspliced XBP1 (XBP1u) and spliced XBP1 (XBP1s) level was detected by Western blot analysis (representative of 3 independent experiments). (C) XBP1u expression was semi-quantified by densitometry (mean ± SD, n = 3).