Fig. 3.

Sal reversed the drug resistance and inhibited HIF-1α signaling pathway. (A-B) Relative 50% inhibitory concentration (IC₅₀) values of DDP/OXA-resistant cells for OXA or DDP in the treatment or co-treatment group. (C-E) IC₅₀ values of PLC/PRF/5, HepG2, and SMMC-7721 cells treated with hypoxia alone and hypoxia combined with Sal. (F) Protein expression level of HIF-1α in cancer cells and OXA-resistant cancer cells. (G) Protein expression level of HIF-1α in PLC/PRF/5 cells treated with hypoxia and hypoxia combined with Sal. (H) Effect of different drugs on the expression levels of HIF-1α in tumor tissues of subcutaneously transplanted tumor mice. (I) Protein expression level of Twist1, Zeb1 and E-cadherin in PLC/PRF/5 cells treated hypoxia and hypoxia combined with Sal. Error bars represent the standard deviation of experiments performed in triplicate (*P < .05, **P < .01).