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. 2018 Dec 20;11:471. doi: 10.3389/fnmol.2018.00471

FIGURE 3.

FIGURE 3

MiR-132 targeted the expression of SOX2 in vitro. (A) SOX2 was predicted as a target gene of miR-132. (B) Dual-Luciferase reporter assay containing the SOX2 wild-type (WT) 3’-UTR or a SOX2 mutant (Mut) 3’-UTR of the SOX2 gene was co-transfected with miR-132 or an empty vector into HEK293T cells, and luciferase activity was assayed. (C) Representative Western blotting bands showed SOX2 expression in neurons after transfections with miR-132 mimic and miR-132 inhibitor. Bar graph showed the SOX2 expression levels relative to β-tubulin. Data are expressed as means ± SEM (n = 6 per group); ∗∗p < 0.01 vs. SOX2 WT3’ -UTR + NC, $p < 0.05 vs. Control; #p < 0.05 vs. OGD.