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. 2018 Dec 20;9:1846. doi: 10.3389/fphys.2018.01846

Table 1.

Management, experimental procedures, and age as well as equivalent implant week of the laying hens of group “Adult” and “Juvenile” in each experimental week.

Experimental week Management Experimental procedures Group “Adult” (20 hens)
Group “Juvenile” (20 hens)
Week of age Implant week Week of age Implant week
1 Arrival at experimental site 21 −3 14 −1
2 B+E 22 −2 15 0
3 B+E 23 −1 16 1
4 Relocation B+E+U 24 0 17 2
5 B+E+U 25 1 18 3
6 B+E+U 26 2 19 4
7 B+E+U+R 27 3 20 5
8 B+E+U 28 4 21 6
9 B+E+U 29 5 22 7
10 B+E+U 30 6 23 8
11 B+E+U 31 7 24 9
12 B+E+U 32 8 25 10
13 B+E+U+CM 33 9 26 11
14 B+E+U+CM 34 10 27 12
15 B+E+U+CM+R 35 11 28 13
16 B+E+U+CM 36 12 29 14
17 B+E+U+CM 37 13 30 15
18 B+E+U+CM 38 14 31 16
19 B+E+U+CM 39 15 32 17
20 B+E+U+CM+D 40 16 33 18

B, blood withdrawal for analysis of estradiol-17ß concentrations; E, health examination; U, ultrasonography of ovaries; CM, comb size measurement; R, radiography of keel bones; D, dissection; implant week 0, administration of deslorelin acetate implant; from this time point on, there were 10 treated and 10 control hens per group.