Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Dec 26;2(1):e201800173. doi: 10.26508/lsa.201800173

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2018 Heuberger et al.

This article is available under a Creative Commons License (Attribution 4.0 International, as described at https://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

Figure 6. — Comparison of control and C/EBPα-depleted small intestinal organoid cultures. C/EBPα KO was induced over 2 consecutive days by administration of 800 nM 4-OHT. (A) Brightfield images of individual tracked control and mutant organoids over a period of 4 d. (B) Measurement of total cell number increase in control and mutant Organoids over a period of 4 d (two-tailed, unpaired t test, n = 9, P < 0.05). (C) Whole-mount IF of EdU-labeled S-phase cells of control (upper) and C/EBPα-depleted (lower) small intestinal organoids. (D) GSEA of RNA sequencing expression data of control and C/EBPα-depleted small intestinal organoids: C/EBPα depletion results in enhanced expression of the E2f, Myc, and Apc target genes and G2M checkpoint genes. KO: C/EBPα-depleted. (E) Quantitative normalized PCR analysis of Ptk6 gene expression in 5 WT and C/EBPα KO organoids with two primer sets, as indicated (two-tailed, unpaired t test, n = 5, P < 0.005). Scale bars: 200 μm.