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. 2018 Dec 14;36(1):25–42. doi: 10.1089/neu.2017.5579

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© Orli Thau-Zuchman et al., 2018; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 4. — Neuroinflammatory response. (A) Images of DAPI, TSPO, Iba-1, and double-labeled TSPO/Iba-1 cells. Note the different microglia morphology (activation): amoeboid versus ramified. Scale bars = 100 μm. To show colocalization, we enlarged the area marked with a rectangle. Scale bars = 100 μm. Immunohistochemistry quantification, around the lesion border, of (B) %Iba-1–positive cells (one-way ANOVA, p = 0.0032; F_(2,11) = 10.1; Bonferroni's post-hoc test, **p < 0.01), (C) %TSPO-positive cells (one-way ANOVA, p < 0.0001; F_(2,11) = 273.7; Bonferroni's post-hoc test, ***p < 0.001), and (D) colocalized TSPO- and Iba-1–positive cells (one-way ANOVA, p < 0.0001; F_(2,11) = 77.97; Bonferroni's post-hoc test, ***p < 0.001). (E) Microglia cell-size analysis (Mann-Whitney U test, **p = 0.0079) and corresponding images, showing the differences in cell size. Insets show the clear morphological differences. Scale bars = 100 μm. Data are means ± SEM of 5 animals/group. ANOVA, analysis of variance; CCI, controlled cortical impact; DAPI, 4’,6-diamidino-2-phenylindole; FC, Fortasyn^® Connect; Iba-1, ionized calcium binding adaptor molecule 1; SEM, standard error of the mean; TSPO, translocator protein.