Synaptic markers. Graph and cropped gels of western blot analysis of protein levels of (A) synaptophysin; 38 kDa (one-way ANOVA, p < 0.0001; F(2,12) = 35.5; Bonferroni's post-hoc test, ***p < 0.001) and (B) PSD-95; 95kDa (one-way ANOVA, p = 0.4188; F(2,12) = 0.9365; Bonferroni's post-hoc test, #p < 0.06) were analyzed by western blot. Data are means ± SEM of 5 animals/group. Neurite outgrowth inhibitor and amyloid load. (C) Graph and cropped gels of western blot analysis of protein levels of Nogo-A; 180 kD (one-way ANOVA, p = 0.0002; F(2,12) = 18.68; Bonferroni's post-hoc test, ***p < 0.001) and (D) β-APP; 87 kDa (one-way ANOVA, p = 0.0163; F(2,12) = 5.915; Bonferroni's post-hoc test, **p < 0.01) were analyzed in both the CCI-FC and craniotomy control mice compared to CCI-control mice. Data are mean ± SEM of 5 animals/group. β-actin was used as loading control. ANOVA, analysis of variance; β-APP, beta-amyloid precursor protein; CCI, controlled cortical impact; FC, Fortasyn® Connect; PSD-95, post-synaptic density 95; SEM, standard error of the mean.