FIG. 8.

Transplanted neural progenitor cells (NPCs)/V2a interneuron (IN) aggregates differentiate into mature neurons and glia. Horizontal section through the lesion/transplant epicenter of a NPC/V2a IN aggregate-transplant showing TdTomato positive V2a INs (A), GFP positive NPCs (B), stained with NeuN (C) to visualize mature neurons, and glial fibrillary acidic protein (GFAP; D) to visualize astrocytes. Overlay of (A-D) is depicted in (E). Black arrowhead points to a mature (NeuN positive) neuron from NPCs, while white arrowheads point to mature (NeuN positive) V2a INs. Red arrowheads point to NeuN negative, TdTomato positive V2a INs. Quantification of the number of V2a INs that were also positive for NeuN as percent of total number of V2a INs counted (F) and as the total number of NeuN positive neurons within GFP positive transplant (G). (H-K) shows a similar section of NPC/V2a IN aggregate, stained for Ki67 (white in J; blue in K), showing proliferative capacity of NPCs, but not V2a INs (white arrowheads in J and K). (L) Confocal orthogonal image of a host (GFP and TdTomato negative) synapse (synaptophysin, blue), onto V2a IN (TdTomato, red). Scale bars are as indicated.