Table 1.
List of Primary and Secondary Antibodies
| Host | Antigen | Concentration | Vendor | Catalogue number |
|---|---|---|---|---|
| Primary antibodies | ||||
| Chicken | GFP | 1:700 IHC | Abcam | Ab13970 |
| Mouse | NeuN | 1:200 IHC | Millipore | MAB377 |
| Mouse | Nestin | 1:1000 IHC | BD Pharmigen | 556309 |
| Mouse | GFAP | 1:400 IHC | Millipore | MAB360 |
| Mouse | Synaptophysin | 1:1000 IHC | Synaptic Systems | 101 011 |
| Mouse | GAD67 | 1:500 IHC | Millipore | MAB5406 |
| Mouse | vGlut1 | 1:1000 IHC | Millipore | MAB5502 |
| Mouse | ENCAM | 1:2 ICC | DSHB | |
| Mouse | A2B5 | 1:2 ICC | DSHB | |
| Rabbit | PRV | 1:10,000 IHC | Courtesy of Lynn Enquist, Princeton University | |
| Rabbit | NeuN | 1:500 IHC | Abcam | Ab177487 |
| Rabbit | Ki67 | 1:200 IHC | ThermoFischer | PA5-16785 |
| Rabbit | ßIII Tubulin | 1:1000 ICC | Abcam | Ab18207 |
| Sheep | CHX10 | 1:200 ICC | Millipore | AB9016 |
| Secondary antibodies | ||||
| Anti-chicken | Alexa Fluor 488 | 1:200 IHC | ThermoFischer | A-11039 |
| Anti-mouse | Alexa Fluor 647 | 1:200 IHC | Molecular Probes | A31571 |
| Anti-mouse | DyLight 405 | 1:200 IHC | Jackson ImmunoResearch | 715-475-150 |
| Anti-rabbit | DyLight 405 | 1:200 IHC | Jackson ImmunoResearch | 711-475-152 |
| Anti-mouse | DyLight 594 | 1:400 ICC; 1:200 IHC | Jackson ImmunoResearch | 715-515-150 |
| Anti-rabbit | Alexa Fluor 488 | 1:400 ICC | Invitrogen/ ThermoFischer | A21206 |
| Anti-sheep | Alexa Fluor 594 | 1:400 ICC | Jackson ImmunoResearch | 713-585-003 |
GFP, green fluorescent protein; IHC, immunohistochemistry; GFAP, glial fibrillary acidic protein; ICC, immunocytochemistry.