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. 2018 Dec 16;21:101078. doi: 10.1016/j.redox.2018.101078

Fig. 4.

Fig. 4

DHEA treatment protects against AGE and AGE-D3T-induced damage. (A) DHEA inhibits AGE and AGE-D3T-induced oxidative stress. (B) G6PD inhibition protects against AGE and AGE-D3T-induced viability loss. Differentiated cells were treated with 100 µM D3T and/or 100 µM DHEA for 24 h prior to 5 mg/mL BSA or AGE treatment. D3T and DHEA were replenished during BSA and AGE treatment. Data were analyzed by one-way ANOVA with Tukey's post-hoc analysis from 3 to 4 independent experiments. Bars with superscript letters different from each other are significantly different (p < 0.05). (C) DHEA treatment protects against AGE and AGE-D3T-induced neurite retraction. (D) DHEA treatment protects against AGE and AGE-D3T-induced loss of long neurites. Differentiated cells were treated with 100 µM D3T and/or 100 µM DHEA for 24 h prior to 5 mg/mL BSA or AGE treatment. D3T and DHEA were replenished during BSA and AGE treatment. A minimum of 100 neurites per group were analyzed. Neurite length and long neurite number in 10x images was quantified using Image J software with NeuronJ plugin. Data were analyzed by one-way ANOVA with Dunnett's post-hoc analysis. Bars with an * are significantly different (p < 0.05) compared to control (BSA).