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. 2018 Nov 27;7(12):488. doi: 10.3390/jcm7120488

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PPARα activates the Nanog promoter. Transcriptional activation of the Nanog promoter by PPARα stimulation in (a) J1 ESCs and (b) 293T cells. J1 ESCs and 293T cells were transfected with the Nanog5P luciferase reporter plasmid and treated with FEN at the indicated concentrations (a and b, left) the next day. Nanog5P (2 μg) was transfected together with pSG5- PPARα (0, 1, 2, and 4 μg, respectively) into J1 ESCs and 293T cells (a and b, right). Firefly luciferase activity was normalized to renilla luciferase activity to correct for transfection efficiencies.