Figure 2.

Metabolic activity of rASCs in osteogenic (OG) cultures treated with metformin. The metabolic activity was monitored after 24 h of culture (d1, A), as well as after 7- (7d, B), 14- (14d, C) and 21- days (d21, D) of culture. Metabolic activity factor was determined in respect to the metabolic activity of the non-osteogenic cultures. Statistical analysis, related to multiple comparisons, was performed with regard to the control culture—metformin non-treated, and was indicate with an asterisk (*). The differences in metabolic activity between rASCs in osteogenic and non-osteogenic cultures were indicated with hashtag (#). The statistical analysis were performed at p < 0.05.