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. 2018 Nov 26;7(12):482. doi: 10.3390/jcm7120482

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Secretory activity of rASCs, both in non-OG and OG cultures. The alkaline phosphatase (ALP) activity was measured in culture supernatants after 14 days of propagation (A). The concentration of osteocalcin (OCL, B), leptin (LEP, C) and bone morphogenetic protein 2 (BMP-2, D) was measured in supernatants collected after 21 day of cultures Statistically significant differences are indicated with an asterisk and hashtag. Statistical analysis, related to multiple comparisons, was performed with regard to the control culture—metformin non-treated, and was indicated with an asterisk (*). The differences in secretory activity of rASCs in osteogenic and non-osteogenic cultures were indicated with hashtag (#). Values are expressed as mean ± SD. Statistically significant differences were noted at p < 0.05 (*/#) and p < 0.001 (###).