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Histology of standardized cranial bone in NCD and CD group. Cranial bones of rats were harvested after four weeks of metformin treatment. Slides are from representative specimens stained with hematoxylin and eosin (H + E). Additionally, the defect sites were visualized using Masson trichrome staining (TM). The osteoclast precursors were detected using the tartrate resistant acid phosphatase (TRAP) staining.
