Table 2.

Major advantages and disadvantages of various high-throughput approaches.

Advantages/Disadvantages	Fluorescence-Based Assay	Luminescence-Based Assay	MS-Based Assay
Advantages	✓Fast and cheap ✓Minimized reagent consumption	✓Fast and cheap ✓Minimized reagent consumption ✓Flexible in the types of tissues used	✓Highly sensitive ✓Accurate
Disadvantages	Probe substrates may be nonselective, thus heterologous- expressed individual CYP should be used Possible fluorescence interference with metabolite by test compounds	Lower activity reading as compared to fluorescence-based assay	Equipment is relatively expensive Need to use internal standard Less high-throughput than fluorescence/luminescence-based assay