. 2018 Dec 8;7(12):200. doi: 10.3390/foods7120200

Table 3.

Mean mesophilic total viable count (TVC_m), psychrophilic total viable count (TVC_p), total Enterobacteriaceae (TEC), Pseudomonas spp., lactic acid bacteria (LAB), Br. thermosphacta, Photobacterium spp. and hydrogen sulphide producing bacteria (HSPB) counts (log₁₀ CFU/cm²) on cod treated with sterile distilled water (SDW), 5% (w/v) citric acid (CA), 5% (v/v) lactic acid (LA), 5% (w/v) capric acid (CP) or 12% (w/v) trisodium phosphate (TSP) and stored at 2 °C for 18 days.

Time (days)	Treatment
Time (days)	CTL ¹		SDW		CA		LA		CP		TSP
TVC_m
	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE
0	3.9	0.2	3.7	0.1	3.7	0.1	3.3	0.4	3.6	0.1	3.4	0.2
2	4.8	0.2	4.9	0.3	4.6	0.4	4.1	0.4	5.2	0.4	4.0	0.4
4	6.1 ^B,2	0.2	6.3 ^B	0.3	5.9 ^B	0.7	5.4 ^A,B	0.4	5.6 ^A,B	0.7	4.6 ^A	0.2
6	6.7 ^A,B	0.4	7.2 ^B	0.2	6.1 ^A,B	0.4	6.7 ^A,B	0.1	6.4 ^A,B	0.5	5.7 ^A	0.2
8	7.1	0.1	7.1	0.2	6.7	0.7	6.8	0.2	7.0	0.3	6.4	0.5
10	7.6	0.3	7.9	0.1	7.8	0.5	7.8	0.1	7.7	0.4	6.9	0.3
12	7.9	0.3	7.1	0.4	7.9	0.1	7.8	0.4	8.0	0.3	7.4	0.4
14	7.9	0.2	7.4	0.3	7.6	0.1	8.2	0.5	7.8	0.5	7.1	0.5
16	8.0	0.1	8.3	0.2	9.0	0.6	8.5	0.5	8.1	0.2	8.2	0.3
18	7.9	0.1	7.9	0.4	8.3	0.3	8.7	0.3	8.3	0.2	8.0	0.4
TVC_p
	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE
0	3.7	0.2	4.0	0.4	3.3	0.3	3.0	0.5	3.2	0.3	2.9	0.2
2	4.6	0.5	5.0	0.5	4.5	0.9	4.3	0.2	4.5	0.8	4.2	0.4
4	6.2 ^B	0.3	6.5 ^B	0.3	5.3 ^A,B	0.9	5.4 ^A,B	0.2	5.3 ^A,B	0.8	4.5 ^A	0.6
6	6.8	0.4	7.2	0.2	6.3	0.8	6.7	0.3	6.3	0.9	5.8	0.6
8	7.6	0.3	7.6	0.2	7.2	0.4	7.1	0.2	7.4	0.4	6.7	0.5
10	7.7	0.4	8.0	0.2	7.9	0.4	7.8	0.4	7.9	0.5	7.1	0.4
12	8.3	0.2	7.7	0.6	8.0	0.3	7.8	0.4	8.6	0.1	7.6	0.2
14	8.4	0.1	7.8	0.4	8.1	0.1	8.3	0.4	8.2	0.1	7.8	0.3
16	8.2	0.0	8.4	0.2	8.9	0.6	8.5	0.2	8.3	0.1	8.2	0.2
18	8.1	0.2	8.1	0.3	8.5	0.2	9.2	0.3	8.6	0.2	8.2	0.1
TEC
	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE
0	1.4	0.7	1.5	0.6	1.2	0.4	1.3	0.6	1.4	0.4	0.4	0.4
2	1.2	0.7	1.6	0.4	1.3	0.5	1.5	0.4	1.3	0.6	1.1	0.1
4	2.2	0.4	2.5	0.3	1.9	0.4	1.9	0.3	2.0	0.5	1.6	0.5
6	2.3	0.6	2.6	0.2	1.5	0.5	2.1	0.7	2.2	0.5	1.6	0.8
8	2.5	0.7	2.6	0.3	2.2	0.7	2.7	0.7	2.4	0.6	2.1	0.8
10	3.0	0.8	3.3	0.4	2.7	0.8	3.3	1.2	2.6	0.6	2.9	0.8
12	2.5	0.5	3.4	0.1	3.1	0.4	2.9	1.2	3.0	0.3	2.7	0.9
14	3.5	0.6	3.4	0.5	3.4	0.7	3.5	0.8	3.6	0.4	2.9	0.7
16	3.6	0.6	3.6	0.4	3.5	0.7	4.1	0.7	3.3	0.4	3.5	1.0
18	3.5	0.6	4.0	0.6	3.6	0.8	4.4	0.7	4.2	0.5	3.8	0.6
Pseudomonas spp.
	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE
0	3.5	0.5	3.6	0.5	3.1	0.5	2.6	0.6	3.3	0.6	2.5	0.5
2	4.8	0.4	5.1	0.2	4.3	0.7	4.5	0.3	4.4	0.6	3.7	0.6
4	6.2 ^A,B	0.3	6.5 ^B	0.3	5.8 ^A,B	0.6	5.3 ^A,B	0.3	6.0 ^A,B	0.4	4.7 ^A	0.2
6	7.0	0.4	7.3	0.2	6.3	0.6	6.9	0.1	6.4	0.7	5.7	0.3
8	7.7	0.3	7.6	0.3	7.5	0.6	7.2	0.3	7.6	0.3	6.8	0.4
10	7.8	0.4	8.5	0.3	8.3	0.4	8.0	0.3	8.1	0.3	7.4	0.5
12	8.1	0.3	8.0	0.4	8.4	0.1	8.2	0.2	8.9	0.2	7.8	0.3
14	8.7	0.3	8.1	0.5	7.9	0.4	8.6	0.4	8.8	0.4	7.9	0.6
16	8.3	0.2	8.8	0.3	9.2	0.9	8.7	0.2	8.6	0.4	8.3	0.3
18	7.8 ^A	0.3	8.2 ^A,B	0.4	8.8 ^A,B	0.3	9.5 ^B	0.6	9.0 ^A,B	0.4	8.7 ^A,B	0.5
LAB
	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE
0	2.6	0.6	2.6	0.5	2.5	0.4	2.0	0.4	2.4	0.4	2.5	0.5
2	2.8	0.5	3.0	0.2	2.6	0.4	2.3	0.4	2.3	0.3	2.5	0.4
4	3.4 ^A,B	0.4	3.8 ^B	0.1	3.1 ^A,B	0.4	2.7 ^A,B	0.4	2.3 ^A	0.4	3.0 ^A,B	0.4
6	4.1 ^B	0.5	4.5 ^B	0.0	3.3 ^A,B	0.5	3.8 ^A,B	0.5	2.8 ^A	0.4	4.0 ^A,B	0.5
8	4.5 ^B	0.6	4.6 ^B	0.2	4.1 ^A,B	0.5	4.5 ^B	0.4	3.2 ^A	0.3	5.1 ^B	0.6
10	4.7 ^B	0.5	5.1 ^B	0.3	4.6 ^B	0.5	5.0 ^B	0.6	3.2 ^A	0.4	5.5 ^B	0.5
12	5.4 ^A,B	0.7	5.5 ^A,B	0.8	5.4 ^A,B	0.3	5.4 ^A,B	1.0	4.4 ^A	0.4	6.2 ^B	0.4
14	5.4 ^A,B	0.4	5.3 ^A,B	0.5	5.0 ^A,B	0.6	5.5 ^A,B	0.7	4.2 ^A	0.8	6.0 ^B	0.2
16	5.5 ^A,B	0.3	5.6 ^B	0.3	5.7 ^B	0.6	6.0 ^B	0.3	4.3 ^A	0.9	6.1 ^B	0.3
18	5.7 ^A,B	0.4	5.9 ^A,B	0.4	6.0 ^A,B	0.3	6.3 ^A,B	0.2	5.0 ^A	1.0	6.5 ^B	0.1
*Br. Thermosphacta*
	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE
0	2.3	0.4	2.3	0.3	1.9	0.4	1.7	0.5	1.5	0.4	1.5	0.4
2	3.3	0.5	3.4	0.2	2.8	0.7	2.8	0.2	2.1	0.6	2.4	0.7
4	4.5 ^B	0.4	5.1 ^B	0.1	3.8 ^B	0.8	3.5 ^A,B	0.4	2.3 ^A	0.4	3.2 ^A,B	0.5
6	5.3 ^B	0.5	5.5 ^B	0.2	4.6 ^B	0.8	5.0 ^B	0.4	2.8 ^A	0.5	4.5 ^B	0.7
8	6.2 ^B	0.5	6.1 ^B	0.3	5.3 ^A,B	0.9	5.3 ^A,B	0.4	3.8 ^A	0.5	5.5 ^B	0.5
10	6.2 ^B	0.4	6.6 ^B	0.4	6.2 ^B	0.5	6.2 ^B	0.3	4.9 ^A	0.1	6.2 ^B	0.6
12	6.7	0.6	6.6	0.6	6.5	0.2	6.2	0.6	5.4	0.0	6.5	0.4
14	6.8 ^B	0.2	6.6 ^A,B	0.3	6.3 ^A,B	0.5	6.5 ^A,B	0.4	5.4 ^A	0.2	6.7 ^A,B	0.2
16	6.9 ^B	0.2	7.2 ^B	0.3	7.0 ^B	0.6	7.0 ^B	0.2	5.2 ^A	0.4	6.9 ^B	0.4
18	7.0	0.2	7.0	0.3	7.0	0.3	7.2	0.2	5.9	0.4	7.3	0.3
Photobacterium spp.
	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE
0	4.1	0.0	3.9	0.1	3.9	0.1	3.3	0.7	3.8	0.0	3.7	0.0
2	4.5	0.2	4.4	0.1	4.3	0.4	4.2	0.2	4.2	0.3	3.7	0.2
4	5.8 ^B	0.1	5.6 ^A,B	0.4	4.8 ^A,B	0.5	5.5 ^A,B	0.3	4.9 ^A,B	0.2	4.1 ^A	0.3
6	6.8	0.6	6.6	0.2	6.1	1.1	6.7	0.4	6.1	1.0	5.4	0.6
8	7.3	0.3	7.2	0.3	6.7	0.8	7.3	0.2	7.2	0.4	6.8	0.7
10	7.3	0.4	7.3	0.3	7.0	0.8	7.7	0.2	7.1	0.7	6.5	0.5
12	8.0	n/a	8.5	n/a	8.2	n/a	8.5	n/a	8.6	n/a	8.0	n/a
14	8.2	0.6	8.0	0.2	7.7	0.6	8.2	0.2	8.0	0.5	7.7	0.7
16	7.9	0.2	8.0	0.1	7.9	0.5	8.3	0.3	8.1	0.3	8.3	0.1
18	8.2	0.2	8.2	0.1	8.9	0.1	9.2	0.4	9.0	0.3	8.9	0.5
HSPB
	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE	Log	SE
0	3.0	0.5	3.1	0.4	2.5	0.5	2.4	0.8	2.0	0.5	2.1	0.2
2	4.4	0.5	4.7	0.3	3.3	1.0	3.6	0.2	3.6	0.9	3.6	0.8
4	5.4 ^A,B	0.1	5.9 ^B	0.2	4.1 ^A	0.8	4.5 ^A,B	0.3	4.4 ^A,B	0.4	4.4 ^A,B	0.4
6	6.4 ^A,B	0.5	7.1 ^B	0.2	5.0 ^A	0.9	5.7 ^A,B	0.3	5.5 ^A,B	0.9	5.7 ^A,B	0.6
8	7.1	0.3	7.1	0.2	5.6	0.8	5.9	0.4	6.2	0.4	6.9	0.4
10	7.2	0.2	7.3	0.4	6.2	1.0	6.0	0.5	6.7	0.6	7.1	0.5
12	7.4	0.3	7.5	0.3	6.8	0.1	6.5	0.2	7.0	0.5	7.6	0.2
14	7.5	0.1	7.4	0.2	6.4	0.3	6.7	0.2	6.9	0.4	7.2	0.2
16	7.6	0.1	7.9	0.3	6.9	0.6	6.9	0.2	6.8	0.5	7.5	0.2
18	7.6	0.1	7.3	0.1	7.0	0.3	7.2	0.3	7.1	0.4	7.6	0.2

¹ CTL = untreated control; ² Statistical analysis: At a given sampling time the same letter indicates no significant (P > 0.05) difference. Absence of a letter also indicates no significant (P > 0.05) difference.