Table 6.
Growth parameters (initial lag period, mean generation time and maximum growth rate (µmax)) of mesophilic total viable count (TVCm), psychrophilic total viable count (TVCp), total Enterobacteriaceae (TEC), Pseudomonas spp., lactic acid bacteria (LAB), Br. thermosphacta, Photobacterium spp. and hydrogen sulphide producing bacteria (HSPB) on cod treated with sterile distilled water (SDW), 1% (v/v) citral (CIT), 1% (v/v) carvacrol (CAR), 1% (w/v) thymol (THY) or 1% (v/v) eugenol (EUG) and stored aerobically at 2 °C for 18 days.
| Bacteria | Initial Lag Time (h) | Mean Generation Time (h) 1 | µmax (Generations h−1) | |||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| CTL 2 | SDW | CIT | CAR | THY | EUG | CTL | SDW | CIT | CAR | THY | EUG | CTL | SDW | CIT | CAR | THY | EUG | |
| TVCm | 24.1 | NA 3 | 21.2 | 29.9 | 50.8 | 24.5 | 18.3 | 17.8 | 14.7 | 12.7 | 13.2 | 15.1 | 0.05 | 0.06 | 0.07 | 0.08 | 0.08 | 0.07 |
| TVCp | 29.2 | NA | 27.6 | 21.8 | 7.0 | 14.4 | 16.3 | 16.3 | 13.0 | 12.5 | 14.5 | 13.7 | 0.06 | 0.06 | 0.08 | 0.08 | 0.07 | 0.07 |
| TEC | 96.6 | 18.7 | 68.6 | 87.7 | NA | 101.1 | 39.0 | 39.4 | 25.8 | 24.4 | 42.4 | 20.7 | 0.03 | 0.03 | 0.04 | 0.04 | 0.02 | 0.05 |
| Pseudomonas spp. | NA | NA | NA | NA | NA | NA | 15.2 | 17.0 | 14.5 | 13.3 | 15.0 | 14.4 | 0.07 | 0.06 | 0.07 | 0.08 | 0.07 | 0.07 |
| LAB | 80.9 | 18.1 | 73.0 | 127.3 | NA | 29.0 | 26.3 | 25.4 | 18.4 | 18.8 | 27.5 | 20.6 | 0.04 | 0.04 | 0.05 | 0.05 | 0.04 | 0.05 |
| Br. thermosphacta | NA | NA | 3.2 | 11.5 | NA | NA | 17.9 | 16.2 | 15.8 | 16.8 | 18.0 | 15.6 | 0.06 | 0.06 | 0.06 | 0.06 | 0.06 | 0.06 |
| Photobacterium spp. | 42.2 | 18.9 | 47.4 | 55.1 | 9.2 | 11.8 | 17.3 | 15.5 | 11.6 | 10.1 | 14.7 | 13.6 | 0.06 | 0.06 | 0.09 | 0.10 | 0.07 | 0.07 |
| HSPB | NA | NA | NA | 78.0 | NA | NA | 19.4 | 17.4 | 11.4 | 12.7 | 15.8 | 15.4 | 0.05 | 0.06 | 0.09 | 0.08 | 0.06 | 0.07 |
1 Calculated using the formula G = t/3.3 logb/B, where t = time interval in h to when the late lag phase was reached, b = number of bacteria at the end of the time interval, and B = number of bacteria at the beginning of the time interval [27]. 2 CTL = untreated control. 3 NA = not applicable as the bacteria were in the log phase of growth at time t = 0.