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. 2018 Nov 27;7(12):489. doi: 10.3390/jcm7120489

Figure 5.

Figure 5

Pretreatment with PT rescued Cr(VI)-induced cytotoxicity. (a) HaCaT cells were pretreated with 10 or 20 μM PT for 1 h. Early apoptosis, detected using an Annexin V apoptosis detection kit, showed that PT (20 μM) inhibited Cr(VI)-induced apoptosis at different concentrations of Cr(VI) (45 and 60 μM) exposure for 24 h. (b) Cell viability was measured using MTT assay. HaCaT cells exposed to Cr(VI) for 24 h after pretreatment with 20 μM PT. PT slightly increased the cell viability of Cr(VI) (60 μM). Data are presented as the mean ± SD from three independent experiments. * p <0.05 versus control; # p <0.05 versus the Cr(VI)-treated group. (c) The expression of apoptosis proteins, pro-caspase 3, cleaved-caspase 3, PARP and GAPDH were monitored after 24 h of Cr(VI) or Cr(VI) plus PT exposure in HaCaT cells. Western blots are representative of n = 3 experiments.