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. 2018 Nov 27;7(12):489. doi: 10.3390/jcm7120489

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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PT attenuates the Cr(VI)-induced pro-inflammatory cytokines and NLRP3 inflammasome. (a,b) The mRNA expressions of TNF-α and IL-1β were measured by real-time PCR. HaCaT cells were treated with several concentrations (7.5, 15, 30, and 45 μM) of Cr(VI) for 12 h. Data presented as the mean ± SD from three independent experiments. * p <0.05 versus controls. (c,d) The expression of the p38 MAPK/MK2 signaling pathway and NLRP3 inflammasome-associated proteins were measured by Western blot analysis following treatment with several concentrations of Cr(VI) for 24 h. (e,f) The cells were pretreated with PT (20 μM) for 1 h and then treated with Cr(VI) (30 μM) for an additional 12 h. Real-time PCR showed that the mRNA levels of pro-inflammatory cytokines, TNF-α and IL-1β decreased after treatment with PT. (g) Western blot analysis demonstrated that the activation of the p38 MAPK/MK2 signaling pathway and the NLRP3 inflammasome was suppressed by PT. Western blots are representative of n = 3 experiments.

PT attenuates the Cr(VI)-induced pro-inflammatory cytokines and NLRP3 inflammasome. (a,b) The mRNA expressions of TNF-α and IL-1β were measured by real-time PCR. HaCaT cells were treated with several concentrations (7.5, 15, 30, and 45 μM) of Cr(VI) for 12 h. Data presented as the mean ± SD from three independent experiments. * p <0.05 versus controls. (c,d) The expression of the p38 MAPK/MK2 signaling pathway and NLRP3 inflammasome-associated proteins were measured by Western blot analysis following treatment with several concentrations of Cr(VI) for 24 h. (e,f) The cells were pretreated with PT (20 μM) for 1 h and then treated with Cr(VI) (30 μM) for an additional 12 h. Real-time PCR showed that the mRNA levels of pro-inflammatory cytokines, TNF-α and IL-1β decreased after treatment with PT. (g) Western blot analysis demonstrated that the activation of the p38 MAPK/MK2 signaling pathway and the NLRP3 inflammasome was suppressed by PT. Western blots are representative of n = 3 experiments.