Table 2.

Primers sequences. Each primer set* was designed to identify all the members of beta-lactamase gene family reported in the GenBank during the 2015 year.

Gene Family	Primer Name	Primer Sequence (5′ to 3′)	Tm (°C)	Position	Amplicon (bp)
blaOXA	BlaOXA-FW	GGTTTCGGTAATGCTGAAATTGG	61.18	214–236	114
	BlaOXA-RW	GCTGTGTATGTGCTAATTGGGA	61.19	327–306
blaVIM	BlaVIM-FW	CGACAGTCARCGAAATTCC	61.39	105–123	133
	BlaVIM-RW	CAATGGTCTSATTGTCCGTG	61.34	238–219
blaSHV	BlaSHV-FW1	CGTAGGCATGATAGAAATGGATC	61.04	133–155	106
	BlaSHV-RW1	CGCAGAGCACTACTTTAAAGG	61.33	239–218
	BlaSHV-FW2	GCCTCATTCAGTTCCGTTTC	61.62	399–418	141
	BlaSHV-RW2	CCATTACCATGAGCGATAACAG	61.22	540–518
blaTEM	BlaTEM-FW	GCCAACTTACTTCTGACAACG	61.80	1699–1719	213
	BlaTEM-RW	CGTTTGGAATGGCTTCATTC	60.13	1912–1892
blaIMP	BlaIMP-FW1	GGAATAGARTGGCTTAAYTCTCG	60.92	319–332	183
	BlaIMP-RW1	CYASTASGTTATCTKGAGTGTG	62.45	502–480
	BlaIMP-FW2	GGTGGAATAGARTGGCTTAAYTC	61.11	316–339	192
	BlaIMP-RW2	CCAAACCACTACGTTATCTKGAG	61.29	508–485
blaROB	BlaROB-FW	CCAACATCGTGGAAAGTGTAG	61.27	718–739	126
	BlaROB-RW	GTAAATTGCGTACTCATGATTGC	60.90	844–821
blaKPC	BlaKPC-FW	GCTAAACTCGAACAGGACTTTG	61.79	100–121	117
	BlaKPC-RW	CTTGAATGAGCTGCACAGTG	61.90	216–197
blaCTX	BlaCTX-FW1	GATACCGCAGATAATACGCAG	60.79	161–181	116
	BlaCTX-RW1	CGTTTTGCGTTTCACTCTG	60.28	276–258
	BlaCTX-FW2	GCTGATTCTGGTCACTTACTTC	61.02	789–810	83
	BlaCTX-RW2	CGCCGACGCTAATACATC	60.69	855–872
	BlaCTX-FW3	CTGCTTAACTACAATCCSATTGC	62.17	314–336	226
	BlaCTX-RW3	GGAATGGCGGTATTKAGC	60.86	539–522
blaCMY	BlaCMY-FW1	GTTTGAGCTAGGATCGGTTAG	60.25	337–357	123
	BlaCMY-RW1	CTGTTTGCCTGTCAGTTCTG	61.48	460–441
	BlaCMY-FW2	GAACGAAGGCTACGTAGCT	61.71	213–231	160
	BlaCMY-RW2	CTGAAACGTGATTCGATCATCA	61.08	372–351
blaDHA	BlaDHA-FW1	GCATATTGATCTGCATATCTCCAC	61.60	399–422	200
	BlaDHA-RW1	GCTGCTGTAACTGTTCTGC	61.62	598–580
	BlaDHA-FW2	GCGGATCTGCTGAATTTCTATC	61.54	464–485	147
	BlaDHA-RW2	GCAGTCAGCAACTGCTCATAC	61.05	610–591
	BlaDHA-FW3	GTAAGATTCCGCATCAAGCTG	61.74	430–450	117
	BlaDHA-RW3	GGGTTATCTCACACCTTTATTACTG	61.08	546–522
blaP	BlaP-FW	GGAGAATATTGGGATTACAATGGC	61.74	271–294	204
	BlaP-RW	CGCATCATCGAGTGTGATTG	61.80	474–455
blaCFX	BlaCFX-FW	CCAGTCATATCATTGACAGTGAG	60.86	437–459	177
	BlaCFX-RW	GACATTTCCTCTTCCGTATAAGC	61.16	613–591
blaLAP	BlaLAP-FW	AGGGCTTGAACAACTTGAAC	61.07	249–268	126
	BlaLAP-RW	GTAATGGCAGCATTGCATAAC	60.59	374–354
blaBIL	BlaBIL-FW	GCCGATATCGTTAATCGCAC	61.65	100–119	128
	BlaBIL-RW	GTTATTGGCGATATCGGCTTTA	60.98	227–206

* All primers were designed in PerlPrimer v1.1.21 running under Debian 8 OS.