Fig. 5.

Levels of extracellular H₂O₂(A) and lipid peroxidation levels (B) in deletion and recombinant yeast strains. The indicated yeast strains were grown in YPD in the presence or absence of 100 µM PQ for 24 h. A. The cells were incubated with Amplex Red and the amount of hydrogen peroxide (µM H₂O₂/10⁹ cells) was calculated as described in the material and methods section. B. The malondialdehyde (MDA) concentration as an indicator for cellular lipid peroxidation was measured and normalized to the total protein amount (µM MDA/mg protein). Given data indicate the ratio of treated (+PQ) to untreated (-PQ) sample (± standard deviation) and are mean values from at least three independent experiments. Values were compared with WT using the unpaired two-tailed t-test; * p-value ≤ 0.05, ** p-value ≤ 0.01, *** p-value ≤ 0.001.