Fig. 6.

Complementation assay with hSco2 variants harboring pathogenic point mutations. A. Scheme of the location of selected pathogenic mutations in the structure of hSco2. The protein backbone of the soluble hSco2 [13] is given in grey. Mutations are shown at their exact positions on the protein structure and are highlighted in different colors. The figure was prepared with PyMOL by rearranging the available structure data retrieved from PDB (PDB: 2RLI). B. Cells of the wild type (WT), the Δsco2Δsod1 mutant strain and its derivatives expressing different hSco2 variants were dropped in a dilution series (10⁴–10¹ cells) onto YPD plates with or without 100 µM PQ. Growth was documented after incubation at 30 °C for three days.