Figure 5.

Dual Pd-NP and C₁₆proMMAE treatment safely blocks tumor growth in multiple tumor models. (a–b) HT1080 (a) and MC38 (b) tumors were treated with Pd-NP, C₁₆proMMAE, or the combination of the two at the indicated time points (red arrows) following tumor formation. Tumor volumes were monitored over time by caliper (n ≥ 6, means ± s.e.m.). (c–d) Changes in individual HT1080 (c) and MC38 (d) tumor volumes were quantified and compared at day 7 (see (a); n ≥ 6, means ± s.e.m; two-tailed Mann–Whitney test). (e–f) Body weight was monitored in animals bearing HT1080 (e) or MC38 (f) tumors following treatment with the combination of Pd-NP and C₁₆proMMAE, showing no significant loss compared to the vehicle control group (n ≥ 4, means ± s.e.m.). (g) At the end of the study end point, plasma of MC38 tumor-bearing mice was analyzed for signs of toxicity, and no significant changes were observed (n ≥ 4, means ± s.e.m.; two-tailed t test at α = 0.05 significance level).