Table 1.
Gene sequences.
| Primer | Forward Sequence | Reverse Sequence | Accession nr/Transcript ID |
|---|---|---|---|
| NCF-1 | TTCATCCGCCACATTGCCC | GTCCTGCCACTTCACCAAGA | NM_001082102.1 |
| CD68 | TTTCCCCAGCTCTCCACCTC | CGATGATGAGGGGCACCAAG | ENSOCUT00000010382 |
| CD11b | TTCAACCTGGAGACTGAGAACAC | TCAAACTGGACCACGCTCTG | ENSOCUT00000001589 |
| CD14 | TCTGAAAATCCTGGGCTGGG | TTCATTCCCGCGTTCCGTAG | ENSOCUT00000004218 |
| ARG1 | GGATCATTGGAGCCCCTTTCTC | TCAAGCAGACCAGCCTTTCTC | NM_001082108.1 |
| IL-4 | CTACCTCCACCACAAGGTGTC | CCAGTGTAGTCTGTCTGGCTT | ENSOCUT00000024099 |
| IL-13 | GCAGCCTCGTATCCCCAG | GGTTGACGCTCCACACCA | ENSOCUT00000000154 |
| M-CSF | GGAACTCTCGCTCAGGCTC | ACATTCTTGATCTTCTCCAGCAAC | ENSOCUT00000030714 |
| OPG | TGTGTGAATGCGAGGAAGGG | AACTGTATTCCGCTCTGGGG | ENSOCUT00000011149 |
| RANKL | GAAGGTTCATGGTTCGATCTGG | CCAAGAGGACAGGCTCACTTT | ENSOCUT00000024354 |
| TRAP | TTACTTCAGTGGCGTGCAGA | CGATCTGGGCTGAGACGTTG | NM_001081988.1 |
| CathK | GGAACCGGGGCATTGACTCT | TGTACCCTCTGCATTTGGCTG | NM_001082641.1 |
| PPAR- γ | CAAGGCGAGGGCGATCTT | ATGCGGATGGCGACTTCTTT | NM_001082148.1 |
| C3 | ACTCTGTCGAGAAGGAACGGG | CCTTGATTTGTTGATGCTGGCTG | NM_001082286.1 |
| C3aR1 | CATGTCAGTCAACCCCTGCT | GCGAATGGTTTTGCTCCCTG | ENSOCUT00000007435 |
| CD46 | TCCTGCTGTTCACTTTCTCGG | CATGTTCCCATCCTTGTTTACACTT | ENSOCUT00000033915 |
| CD55 | TGGTGTTGGGTGGAGTGACC | AGAGTGAAGCCTCTGTTGCATT | ENSOCUT00000031985 |
| CD59 | ACCACTGTCTCCTCCCAAGT | GCAATCTTCATACCGCCAACA | NM_001082712.1 |
| C5 | TCCAAAACTCTGCAACCTTAACA | AAATGCTTTGACACAACTTCCA | ENSOCUT00000005683 |
| C5aR1 | ACGTCAACTGCTGCATCAACC | AGGCTGGGGAGAGACTTGC | ENSOCUT00000029180 |
| CD3 | CCTGGGGACAGGAAGATGATGAC | CAGCACCACACGGGTTCCA | NM_001082001.1 |
| CD4 | CAACTGGAAACATGCGAACCA | TTGATGACCAGGGGGAAAGA | NM_001082313.2 |
| CD8 | GGCGTCTACTTCTGCATGACC | GAACCGGCACACTCTCTTCT | ENSOCUT00000009383 |
| CD19 | GGATGTATGTCTGTCGCCGT | AAGCAAAGCCACAACTGGAA | ENSOCUT00000028895 |
| GAPDH | GGTGAAGGTCGGAGTGAACGG | CATGTAGACCATGTAGTGGAGGTCA | NM_001082253.1 |
| ACT-β | TCATTCCAAATATCGTGAGATGCC | TACACAAATGCGATGCTGCC | NM_001101683.1 |
| LDHA | TGCAGACAAGGAACAGTGGA | CCCAGGTAGTGTAGCCCTT | NM_001082277.1 |
NCF-1, neutrophil cytosolic factor 1; CD68, macrosialin; CD11b, macrophage marker; CD14, monocyte differentiation antigen CD14; ARG1, Arginase 1; IL-4, Interleukin 4; IL-13, Interleukin 13; M-CSF, colony stimulating factor-macrophage; OPG, osteoprotegerin; RANKL, Receptor activator of nuclear factor kappa-B ligand; TRAP, tartrate resistant acid phosphatase; CathK, cathepsin K; PPAR-γ, peroxisome proliferator activated receptor gamma; C3, complement component 3; C3aR1, complement component 3a receptor 1; CD46, complement regulatory protein; CD55, decay accelerating factor for complement; CD59, complement regulatory protein; C5, complement component 5; C5aR1, complement component 5a receptor 1; CD3, T-cell surface glycoprotein CD3; CD4, T-cell surface glycoprotein CD4; CD8, T-cell transmembrane glycoprotein CD8; CD19, B-lymphocyte surface protein CD19; GAPDH, glyceraldehyde-3-phosphate dehydrogenase; ACT-β, actin beta; LDHA, lactate dehydrogenase A.