Figure 5.

BPA increases BMAL1 binding to the Npy promotor. (A) Schematic representing the 103-bp region of the Npy promotor, containing two potential BMAL1 binding sites (gray ovals), amplified for the ChIP assay with qRT-PCR. (B) mHypoA-Bmal1-WT/F cells were treated with 100 µM BPA or vehicle (0.05% ETOH) for 4 hours, and changes in BMAL1 binding to the −1267 to −1165 region of the Npy promotor were measured with a ChIP assay (n = 3). A vehicle-treated chromatin sample was incubated with normal IgG antibody as a negative control. Data are expressed as mean ± SEM. Statistical significance was determined with a one-way ANOVA, followed by the Bonferroni multiple comparison test. *P < 0.05. F, forward; R, reverse.