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Efficient Gene Activation Using dSpCas9VPR in HEK293A Cells
qPCR analysis of gene activation for (A) OTX2, (B) RAX, (C) RORB, (D) CRX, (E) ASCL1, NEUROD1, or multiplex induction of ASCL1 and NEUROD1. Results are displayed as the mean of three independent biological repeats ± SEM. (F) Immunocytochemistry results showed upregulated protein expression of ASCL1 (green) in HEK293A following gene activation with dSpCas9VPR. Scale bars, 100 μm.
