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. 2018 Nov 20;14:184–191. doi: 10.1016/j.omtn.2018.11.008

Figure 4.

Figure 4

Comparison of Multiple sgRNAs to Induce Gene Activation in Rat Müller Glial Cell rMC1 Using dSaCas9VPR

qPCR analysis of gene activation for (A) Ascl1 using two sgRNAs, (B) Neurod1 using two sgRNAs, and (C) Nrl using three sgRNAs. Results are displayed as the mean of three to five independent biological repeats ± SEM.