Table 1.

Information of sgRNAs Used for SpCas9

Species	Name	TSS Distance	Strand	Sequence	PAM	On-Target Score
Rat	Ascl1 sgRNA 1	−183 bp	+	5′-ACGCACTGCAACAACAAACC-3′	CGG	46.1
	Ascl1 sgRNA 2	−351 bp	−	5′-TCCTAGGTAGAAAGTCTGGA-3′	GGG	73.4
	Neurod1 sgRNA 1	−258 bp	+	5′-TGCGGGTAAAAACAGGTCCG-3′	CGG	56.1
	Neurod1 sgRNA 2	−164 bp	+	5′-ATACAAATAGGCAGGTCACG-3′	TGG	84.1
	Nrl sgRNA 1	−573 bp	−	5′-CTTTACCTCTCAAAGCCTTC-3′	AGG	27.5
	Nrl sgRNA 2	−764 bp	+	5′-CCATCTGCTTAGACTCACCA-3′	TGG	77.8
Human	ASCL1 sgRNA 1	−181 bp	+	5′-CGGGAGAAAGGAACGGGAGG-3′	GGG	30.9
	NEUROD1 sgRNA 1	−33 bp	+	5′-AGGGGAGCGGTTGTCGGAGG-3′	AGG	30.9
	RAX sgRNA	−99 bp	+	5′-GAGGGAGGGGCCGAGAGAAG-3′	GGG	44.0
	OTX2 sgRNA	−170 bp	+	5′-AGATTGTAATTGCTTTCTTC-3′	GGG	35.2
	CRX sgRNA	−114 bp	−	5′-AGGGAGGCCCCAGCTCCTGC-3′	CGG	51.6
	RORB sgRNA	−172 bp	+	5′-CCCGGCCACCTCGGACTCCC-3′	TGG	41.8
Mouse	Nkx2.5 sgRNA	−171 bp	+	5′-GTATTTTCTTTGAGTGTGTC-3′	TGG	36.1

On-target score is an optimized score for 20-bp sgRNA based on Doench et al.²⁸. Scores range from 0–100. TSS, transcription start site.