Table 2.
Information of sgRNAs Used for SaCas9
| Species | Name | TSS Distance | Strand | Sequence | PAM | On-Target Score | On-Target Score Ranking |
|---|---|---|---|---|---|---|---|
| Rat | Ascl1 sgRNA 1 | −180 bp | + | 5′-GCACTGCAACAACAAACCCGG-3′ | CTGAAT | 70.9 | 1 |
| Ascl1 sgRNA 2 | −223 bp | − | 5′-TGGCGCGTGCCGGACTCCCGG-3′ | CTGAAT | 64.2 | 5 | |
| Neurod1 sgRNA 1 | −258 bp | + | 5′-CTGCGGGTAAAAACAGGTCCG-3′ | CGGAGT | 56.1 | 2 | |
| Neurod1 sgRNA 2 | −205 bp | + | 5′-TTCTTCTGGCCACAAAGGGGC-3′ | CGGAAT | 38.5 | 5 | |
| Nrl sgRNA 1 | −575 bp | − | 5′-TTTACCTCTCAAAGCCTTCAG-3′ | GAGAGT | 79.3 | 1 | |
| Nrl sgRNA 2 | −145 bp | + | 5′-TTCAGGGCTGCTTCATTACTC-3′ | CGGAAT | 45.1 | 9 | |
| Nrl sgRNA 3 | −760 bp | − | 5′-TTTAACTTAGCACCTGCCATG-3′ | GTGAGT | 72.7 | 2 | |
| Human | ASCL1 sgRNA | −239 bp | + | 5′-GCACTGCAACAACAAACCCAG-3′ | CTGAAT | 74.2 | 2 |
On-target score is an optimized score for 20-bp sgRNA based on Doench et al.28. The on-target scores are ranked for sgRNAs targeting 2,000-bp upstream of transcription start site of genes. Scores range from 0–100. TSS, Transcription start site.