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. 2018 Dec 27;19:973. doi: 10.1186/s12864-018-5353-y

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — ampC copy number for different strains carrying an ampC amplification. The ampC copy number was determined with qPCR, using untreated wild-type E. coli as a reference. With exception of wild-type (which only acquired resistance to amoxicillin), all strains carried a previous resistance to enrofloxacin (ENRO_R), kanamycin (KAN_R), or tetracycline (TET_R), resulting in a secondary resistance to amoxicillin The number displayed under the strain indicates the concentration amoxicillin used for resistance development. Bars indicate the average copy number from 25 colonies