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. 2018 Dec 27;18:81. doi: 10.1186/s12896-018-0492-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Mxr1-reprogrammed cells were induced under broad conditions. a AOX activity in different cells in response to different carbon sources. b After being cultured in BMGY (2% glycerol), cells were concentrated in the original medium, or the medium was replaced by fresh medium without residual glycerol. The cells were then induced with 0.5% methanol per day. The results represent the mean of three independent biological replicates. Two-way ANOVA and the least significant difference (LSD) test were used to determine significance (p = 0.05). c After being cultured in BMGY (2% glycerol), cells were cultured in BMGY and induced with 0.33% glycerol per day. The error bars represent the standard deviation of three biological replicates. The independent-sample t-test was used to determine significance. NS, p > 0.05; ***, p < 0.005