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. 2018 Dec 27;18:81. doi: 10.1186/s12896-018-0492-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 5 — Synthetic circuit of Mxr1 enhanced scFv expression. After being cultured in BMGY (1% glycerol), cells were cultured in BMMY and induced with 1% methanol daily. a The relative secreted scFv titre is presented. The error bars represent the standard deviation of three biological replicates. One-way ANOVA and the Tukey test were used to determine significance. *, p < 0.05; ***, p < 0.005. b Intracellular proteins after induction were examined by western blotting using an anti-his antibody. GAPDH was used as the loading control