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. 2018 Dec 27;18:81. doi: 10.1186/s12896-018-0492-4

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© The Author(s). 2018

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 6 — The schematic diagram of transcription regulation in Mxr1-reprogrammed cells. There are two phases, derepression and activation, for P_AOX1 activation in P. pastoris. Prm1, Mit1, and Mxr1 are activators (blue). Nrg1 and X are repressors (red). The dashed line and repressor X are speculated based on our results, for which further investigation is needed. In reprogrammed cells, we used P_AOX2 to express extra Mxr1 (dark blue square)