Fig. 7.

TRIF-interferon-GBPs pathway is required for caspase-11 activation in vitro. a Western-blot to detect the expression of caspase-11, IL-1β and β-Actin in splenocytes isolated from mice of indicated genotypes following intraperitoneal injection with either 10 mg/kg LPS or saline. b-d ELISA for IL-1α, IL-1β and LDH assay in the supernatants of peritoneal macrophages stimulated with either LPS (1 μg/ml) alone or LPS (1 μg/ml) plus CTB. Supernatants were collected 16 h after stimulation. e Western-blot for Na+/K+ ATPase, Rab7, LAMP1 and β-actin in the cytosolic and residual fractions from LPS (1 μg/ml) or CTB plus LPS (1 μg/ml) or CTB alone -stimulated mouse peritoneal macrophages obtained by digitonin fractionation. f LPS activity assay in the cytosolic and residual fractions from LPS (1 μg/ml) or CTB plus LPS (1 μg/ml) or CTB alone -stimulated mouse peritoneal macrophages obtained by digitonin fractionation.*P<0.05; **P<0.01; ***P<0.001 (Student’s t-test). Graphs show the mean ± SEM. Data are representative of at least three independent experiments