Fig. 2.

Leukocyte infiltration in the tubulointerstitium. Leukocyte subsets were immunohistochemically detected in kidney biopsy samples using specific antibodies (Additional file 2: Table S3: Primary antibodies) and scored according to Table 2. Neutrophils (a, b), macrophages (d, e), T lymphocytes (g, h), and B lymphocytes (j, k) were observed in the interstitium in kidney samples from control patients (a, d, g, j) and patients with sepsis (b, e, h, k), and mean leukocyte counts were determined (c, f, i, l). T lymphocytes, B lymphocytes, and macrophages were quantified using an arbitrary score (0 = absent, 1 = focal, 2 = diffuse; c, f, i). For neutrophils, absolute cell counts were determined per high power field (HPF). Black arrows show stained leukocytes of various subsets. *Statistically significant difference. Black lines are medians (c, f, i, l). Red scale bars = 50 μm