Fig. 7.

miR-584-5p regulates the expression of MSRB3 in pigs. a A target site of miR-584-5p was identified in the 979–1000 bp region downstream of the stop codon of the MSRB3 gene via DIANA-microT web server (v5.0). b Comparison of the expression levels of miR-584-5p between CNV/CNV and wild-type animals. c eQTL mapping for miR-584-5p in ear tissue samples. The CNV shows the strongest association with miR-584-5p expression. d Construction of the mutant 3′UTR reporter. The 7-bp seed region (in bold) was deleted in the mutant 3′UTR reporter. e Porcine fetal fibroblast cells were transfected with the 3′UTR mutant or wild-type luciferase reporters and co-transfected with miR-584-5p mimics or the negative control duplexes. The relative luciferase activity was measured 48 h later. The figure shows the mean ± S.D. from three independent experiments performed in duplicate (**, P < 0.01). The MSRB3 mRNA expression levels (f) and protein levels (g) in porcine fetal fibroblast cells transfected with miR-584-5p mimics or negative control duplexes