Figure 2.

Lentiviral miR-138 Increase Proliferation and Cytotoxicity of γδ T Cells without Influence on EV Production

(A) miR-138 levels in γδ T cells were measured by qRT-PCR. Data represent at least three experiments done in triplicate. (B) The absolute cell number of γδ T cells after lentiviral miR-138 transfection and subsequent expansion. (C) Absolute number of γδ T cells at the indicated time points. Data represent at least three experiments done in triplicate. (D) The cytotoxicity of γδ T cells against cancer cells was measured by an LDH cytotoxicity assay with an E:T ratio of 10:1. Data represent at least three experiments done in triplicate. (E) Quantification of EVs derived from scramble miRNA or miR-138-transfected γδ T cells. Data represent at least three experiments done in triplicate. (F) miR-138 levels in γδTDEs were measured by qRT-PCR. Data represent at least three experiments done in triplicate. *p < 0.05.