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. 2018 Nov 30;17(1):679–688. doi: 10.3892/etm.2018.7036

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Copyright: © Wang et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 2. — Dex/vitD3-treated DCs induce the generation of IL-10-producing T cells. (A-D) The mRNA levels of T cell polarized transcription factors (including T-bet, Gata-3, RORγt and Foxp3) were detected. Results are presented as the mean concentration ± standard error of the mean of six independent experiments. (E) Summary of six independent experiments. (F) Intracellular staining of cytokine production assessed by flow cytometry. These data provided the percentage of IFN-γ-, IL-17- and IL-10-producing cells in T [DC], T [DC + LPS] or T [DC + Dex/vitD3 + LPS] cells. A representative experiment out of six is presented. **P<0.01. Dex, dexamethasone; vitD3, vitamin D3; DC, dendritic cell; IL, interleukin; T-bet, T-box 21; Gata-3, GATA binding protein 3; RORγt, RAR related orphan receptor γ 2; Foxp3, forkhead box P3; LPS, lipopolysaccharide; IFN-γ, interferon-γ; T, T cells.

Figure 2. — Dex/vitD3-treated DCs induce the generation of IL-10-producing T cells. (A-D) The mRNA levels of T cell polarized transcription factors (including T-bet, Gata-3, RORγt and Foxp3) were detected. Results are presented as the mean concentration ± standard error of the mean of six independent experiments. (E) Summary of six independent experiments. (F) Intracellular staining of cytokine production assessed by flow cytometry. These data provided the percentage of IFN-γ-, IL-17- and IL-10-producing cells in T [DC], T [DC + LPS] or T [DC + Dex/vitD3 + LPS] cells. A representative experiment out of six is presented. **P<0.01. Dex, dexamethasone; vitD3, vitamin D3; DC, dendritic cell; IL, interleukin; T-bet, T-box 21; Gata-3, GATA binding protein 3; RORγt, RAR related orphan receptor γ 2; Foxp3, forkhead box P3; LPS, lipopolysaccharide; IFN-γ, interferon-γ; T, T cells.