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Specificity of the PCR assay for detecting the target gene of hypothetical protein gene. Genomic DNA of P. aeruginosa was used as the template for PCR in lanes 1–8, the template in lanes 9–14 were ordinal of control strains, E. coli, K. pneumonia, S. aureus, S. epidermidis, E. faecalis and S. bacillus, whereas lane 15 is NC. All experiments were repeated twice. PCR, polymerase chain reaction; NC, negative control; M, marker.
