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Sensitivity of the polymerase chain reaction assay for detection of the hypothetical protein gene. The positive plasmid was used as a template at concentrations of 5×108, 5×107, 5×106, 5×105, 5×104, 5×103, 5×102, 5×101 and 5×10° per reaction, with nuclease-free water used as template for NC. All experiments were repeated six times. NC, negative control; M, marker.
