Figure 4.
Sensitivity of the loop-mediated isothermal amplification assay for detection of the hypothetical protein gene. (A) The positive plasmids as template at concentrations of 5×109, 5×108, 5×107, 5×106, 5×105, 5×104, 5×103, 5×102, 5×101 and 5×10° per reaction, with nuclease-free water as template in lane 1 for NC. (B) The blood sample being direct lysed as template at concentrations of 1×108, 1×107, 1×106, 1×105, 1×104, 1×103, 1×102, 1×101 and 1×10° per reaction the sample order from 1×10°−1×108, respectively, with positive plasmids as the template in lane 11 as PC, and nuclease-free water as the template in lane 12 as NC. All the experiments were repeated six times. PC, positive control; NC, negative control; M, marker.