Figure 4.

miR675 Affects the Interaction among hMSH6, H3k36me3, and SKP2 in the Human Mesenchymal Stem Cells Infected with rLV and rLV-miR675, Respectively

(A) Anti-STED2 co-immunoprecipitation (coIP) followed by western blotting with anti-P62 and anti-histone 3. IgG IP was the negative control. INPUT refers to western blotting with anti-STED2. (B) Anti-SETD2 coIP followed by western blotting with anti-histone 3. IgG IP was the negative control. INPUT refers to western blotting with anti-SETD2. (C) (a) Western blotting with anti-H3K36me1, anti-H3K36me2, and anti-H3K36me1. β-actin was the internal control. (b) The gray scan analysis of positive bands of western blotting. (D) Anti-hMSH6 coIP followed by western blotting with anti-H3K36me3 and anti-SKP2. IgG IP was the negative control. INPUT refers to western blotting with anti-hMSH6. (E) Anti-hMSH6 coIP followed by western blotting with anti-SKP2 in the human mesenchymal stem cells, including the rLV and rLV-miR675 plus pCMV6-AC-GFP-JMJD2A groups. IgG IP was the negative control. INPUT refers to western blotting with anti-hMSH6.