Skip to main content
. 2018 Nov 24;14:171–183. doi: 10.1016/j.omtn.2018.11.010

Figure 5.

Figure 5

miR675 Delays the hMSH6-H3k36me3-Skp2 Ternary Complex Occupancy on the Mismatch DNA in the Human Mesenchymal Stem Cells Infected with rLV and rLV-miR675, Respectively

(A) (a) Super-EMSA (gel shift) with biotin-DNA probe (including mismatch and match DNA double strands) and anti-histone H3.3 antibody. The intensity of the band was examined by western blotting with anti-biotin. (b) The gray scan analysis of positive bands of super-EMSA. (B) Biotin-mismatch probe pull-down followed by western blotting with anti-histone 3.3, anti-histone 3, anti-SKP2, anti-hMSH6, and anti-H3K36me3. Biotin was the INPUT and histone was the internal control. (C) Chromatin immunoprecipitation (ChIP) with anti-histone 3.3, anti-histone H3, anti-SKP2, anti-hMSH6, and anti-H3K36me3 followed by PCR with damaged DNA primers. IgG ChIP was the negative control and damaged DNA was the INPUT. (D) Biotin-match double DNA probe pull-down followed by western blotting with anti-histone H3.3, anti-histone H3, anti-SKP2, anti-hMSH6, and anti-H3K36me3. Biotin was the INPUT and histone was the internal control. (E) ChIP with anti-histone 3.3, anti-histone H3, anti-SKP2, anti-hMSH6, and anti-H3K36me3 followed by PCR with match double DNA primers. IgG ChIP was the negative control and match double DNA was the INPUT.