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. 2018 Nov 24;14:171–183. doi: 10.1016/j.omtn.2018.11.010

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© 2018 The Authors

This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).

PMC Copyright notice

miR675 Inhibits DNA Damage Repair and Decreases Aging Histone H3.3 Degradation in the Human Mesenchymal Stem Cells Infected with rLV, rLV-miR675, and rLV-miR675 Plus rLV-Cas9-P62, Respectively

(A) Restriction endonuclease analysis with BamHI and EcoRI for plasmid DNA injury repair. (B) (a) Anti-SKP2 coIP followed by western blotting with anti-histone 3.3. IgG IP was the negative control. INPUT refers to western blotting with anti-histone H3.3. (b) The gray scan analysis of positive bands. (C) Anti-Ub coIP followed by western blotting with anti-histone H3.3. IgG IP was the negative control. INPUT refers to western blotting with β-actin.