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. 2018 Oct 30;17(1):260–272. doi: 10.3892/etm.2018.6902

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Copyright: © Muhammad et al.

This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.

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Figure 3. — miR-30d inhibits cell growth ability in vitro. (A) RT-qPCR was performed to determine the relative expression levels of miR-30d in five colon cancer cell lines. (B) miR-30d mimic or NC mimic and inhibitor or NC inhibitor were used to transiently transfect SW480 and HCT116 cells, respectively. RT-qPCR was performed to determine the expression of miR-30d after 24 h. (C and D) An MTT assay was performed to examine the effects of miR-30d on proliferation. (C) *P<0.05 vs. mimic; (D) *P<0.05 vs. inhibitor. (E) A colony formation assay was performed to determine the effects of miR-30d on proliferation. (F) The number of clones was analyzed quantitatively. *P<0.05, **P<0.01. Data are presented as the mean ± standard error of the mean. RT-qPCR, reverse transcription-quantitative polymerase chain reaction; miR, microRNA; NC, negative control.