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. 2018 Oct 30;17(1):260–272. doi: 10.3892/etm.2018.6902

Figure 6.

Figure 6.

Tumor suppressive effects of miR-30d via GNA13 expression inhibition. (A and B) Expression of (A) miR-30d expression and (B) GNA13 was evaluated by RT-qPCR and western blot analysis, respectively, in SW480 cells co-transfected with miR-30d mimic (or NC mimic) and GNA13 [or pcDNA3.1(+)]. *P<0.05 GNA13 vs. pcDNA3. (C-E) Transwell migration and invasion assays. (C) Representative images (magnification, ×200) of Transwell assays; (D and E) quantitative analysis of migration and invasion. *P<0.05, **P<0.01. (F) SW480 cells were transfected with siGNA13 or siNC. GNA13 expression was determined by western blotting after 48 h. (G and H) Following transfection with siRNA or siNC, Transwell migration and invasion assays were performed. (G) Representative images (magnification, ×200) of Transwell assays; (H) quantitative analysis of migration and invasion. *P<0.05, **P<0.01 vs. siNC. miR, microRNA; NC, negative control; RT-qPCR, reverse transcription-quantitative polymerase chain reaction; siRNA or si, small interfering RNA.