Inhibitory proliferation effects of SSd in 143B and MG-63. (A) MTS analysis was performed to detect proliferation at various SSd concentrations (20, 40, 60 and 80 µmol/l) following 24 and 48 h treatment at a wavelength of 450 nm. (B) EdU assays were performed to measure 143B and MG-63 viability at various SSd concentrations (20, 40, 60 and 80 µmol/l) following 24 and 48 h treatment. ***P<0.001 vs. control. SSd, saikosaponin D; OD, optical density.