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. 2018 Dec 20;9:2966. doi: 10.3389/fimmu.2018.02966

Figure 1.

Figure 1

Confocal microscopy images of live human uterine smooth muscle cells following application of rhodamine-conjugated CPPs or CPP-cargo combinations. Images captured 60 min following addition of either 1, 3, or 10 μM concentrations of (A) Rhodamine—Penetratin, (B) Rhodamine—Pen-NBD, or (C) Rhodamine—Pen (43-56)-NBD. (D) Displays cells imaged 60 min following addition of 10 μM control peptide Rhodamine - GS4 (GC). Scale bars 20 μm. Cells are maintained in serum deprived media within a temperature-controlled chamber (37°C, 5%CO2). To demonstrate intra-cellularity of uptake, images are taken from the center of a Z-stack of 3–10 slices (0.54 μm apart) intended to capture the full depth of the cell. Images are representative of 3 independent experiments.