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. 2018 Dec 20;9:2966. doi: 10.3389/fimmu.2018.02966

Figure 4.

Figure 4

Effect of Pen-NBD on the cytokine–induced degradation of IκBα protein and phosphorylation of P65 protein. (Left) Representative Western blots demonstrating responses of (A) IκBα protein and (B) phosphorylated P65 protein at 15 and 60 min following application of 10 ng/ml IL1β either alone or following pre-incubation for 1 h with indicated concentration of Pen-NBD peptide prior to IL1β addition. Actin expression displayed as loading control. Ø = no protein loaded. (Right) Scatter plots demonstrating raw optical density values of (A) IκBα protein and (B) phosphorylated P65 protein signal at 15 and 60 min time points comparing IL1β alone or 100μM Pen-NBD plus IL1β experiments. *Significant difference from raw optical density values between IL1β alone or 100 μM Pen-NBD plus IL1β groups (n = 4, two-way ANOVA with Sidak's post-hoc correction).